despite the commercial availability of several systems for wastewater treatment, many companies haven’t plants, and others have inefficient systems, whereby, discharged into water bodies significant amounts of organic matter and microorganisms related to the substrate, as coliforms, endangering the environmental balance. this study evaluated the ability of a mixture of beneficial microorganisms (bm) to remove coliforms and e. coli present in liquid waste generated in a dairy wastewater treatment plant, having as variables, the concentration of bm cocktail and stored organic loading rate in influent. reduction in coliform population fluctuated between 41.1 and 48% when using different concentrations of bm for hours making set. as for the elimination of e. coli, notable decreases were observed from samples taken at 10 o′clock, with a 52% reduction when using the cocktail to 2% and 49.7% when the concentration of that mixture corresponded to 4%. it is recommended to improve the efficacy in removing coliforms and e. coli from the evaluation of several ratios between the concentrations of the constituents of the mixture.