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The use of botanical microtechnique paraffin in anatomical studies of lichens

DOI: 10.2478/v10067-008-0007-0

Keywords: microtechnique paraffin, microscopic slides, fruticulous lichens: Evernia prunastri, Ramalina fastigiata, Sulcaria sulcata, Usnea florida, U. subfloridana

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Abstract:

The paper describes a simple method of making paraffin slides from dry and unfixed lichen specimens, several methods of their staining and assessment of color stability of used dyes. It was demonstrated for the first time that lichen sections from permanent slides can be hydrated and their structure characteristic of fresh material can be restored.

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