Objectives; To assess the staffing gap by cadre in the health facilities, determining the proportion of health workers motivated and assessing the factors associated with their motivation Study design; Descriptive cross sectional study Settings; Moshi Rural district Results; It was found that the staffing gap were 42.3%, 38.2% and 46.2% in the hospitals, health centres and dispensaries respectively. Comparison was made between the public, private and religious health facilities. It was found that 21.2%, 62.5% and 95.8% of health workers from public, private and religious health facilities respectively reported of presence of enough working equipment in their health facilities. In addition 36.1%, 54.3% and 81.6% of health workers from public, private and religious health facilities were being promoted when deserving. From the information above it is evident that health workers from religious health facilities have the highest level of motivation, those from the private health facilities are moderately motivated while those from public health facilities are poorly motivated. Conclusion and Recommendations Motivation of health workers and availability of working equipments in Moshi rural is highest in religious health facilities, moderate in private health facilities and lowest in public health facilities . Efforts should be made to find affordable means of motivating the rural health workers so as to improve their performance and ultimately the health status of Tanzanians.