byrsonima sericea leaves are extensively used in folk medicine in brazil against gastric disorders. this study investigated the chemical constituents of b. sericea leaf ethanolic extract (bsle) and its potential gastroprotective activity, with its possible mechanism of the action using ethanol to induce gastric mucosal damage in mice. the phytochemical analysis was carried out to identify the active constituents present in the extract, and the hplc analysis was performed for the identification of flavonoids. bsle at oral doses of 125, 250 and 500 mg/kg markedly attenuated the ethanol-evoked gastric lesions by 53.2, 84.9 and 87.6 %, respectively. the bsle (250 mg/kg) prevented the depletion of gastric mucus and gastric mucosal nonproteic-sulfhydryl groups, sod and cat, as well as the increase in the mda content promoted by absolute ethanol. moreover, the effect of bsle against ethanol damage was found to be significantly reduced in mice pretreated with capsazepine (i.p.), l-name (i.p.) or glibenclamide (i.p.), the respective blockers/inhibitors of trpv1, no synthase and k+atp channel. the phytochemical investigation on bsle revealed the presence of flavonoids rutin, isoquercitrin, kaempferol 3-o-rutinoside and quercetin, which are compounds well known for their antioxidant and gastroprotective properties. these results suggest that bsle affords gastroprotection through multiple mechanisms, which may be helpful in the treatment of pathologies associated with gastric dysfunctions.