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Insulina pancreática de ratos diabéticos tipo 1 submetidos a um protocolo de treinamento físico individualizado Pancreatic insulin of type I diabetic rats subjected to an individualized exercise-training protocol
Leandro Pereira de Moura,Ricardo José Gomes,José Almeida Leme,Fabrício Azevedo Voltarelli
Motricidade , 2012, DOI: 10.6063/motricidade.8(1).234
Abstract: Dado que o diabetes mellitus é considerado um problema de saúde pública mundial, o presente estudo objetivou avaliar a tolerancia à glicose oral (TTGo) e concentra es de insulina pancreática (CIP) de ratos diabéticos tipo 1 submetidos a um protocolo de treinamento físico individualizado. Foram utilizados 40 ratos Wistar adultos, onde metade foi induzida ao diabetes por meio de inje o endovenosa de aloxana (32 mg/kg), divididos em quatro grupos (10 por grupo): Controle Sedentário (CS), Controle Treinado (CT), Diabético Sedentário (DS) e Diabético Treinado (DT). O treinamento físico consistiu de nata o, 1 h/dia, 5 dias/semana durante 8 semanas, com sobrecarga equivalente a 90% da transi o metabólica aeróbia/anaeróbia determinada no início do experimento. Os ratos dos grupos DS e DT apresentaram redu o de peso corporal em rela o aos controles, a qual foi menos acentuada no grupo DT. As ingest es hídrica e alimentar aumentaram nos grupos diabéticos em rela o aos controles. Durante o TTGo, os valores de área sob a curva glicêmica dos grupos diabéticos foram superiores aos dos controles. O treinamento físico atenuou esta eleva o. Os grupos diabéticos apresentaram CIP reduzida quando comparados aos grupos controles. O protocolo de treinamento físico empregado, embora n o tenha alterado a CIP, melhorou a homeostase glicêmica e atenuou a perda de peso corporal dos animais diabéticos. Since diabetes mellitus is considered a world public health problem, the present study aimed to evaluate oral glucose tolerance (TTGo) and pancreatic insulin concentration (PIC) of type I diabetic rats subjected to an individualized exercise training protocol. A total of 40 adult Wistar rats were used, half of which induced to diabetes by alloxan (32 mg/kg) endovenous injection, and divided into four groups (10 per group): Sedentary Control (SC), Trained Control (TC), Sedentary Diabetic (SD) and Trained Diabetic (TD). The physical training consisted of swimming, 1 h/day, 5 days/week during 8 weeks, supporting overload equivalent to 90% of the individual anaerobic/aerobic metabolic transition determined at the beginning of the experiment. The rats of SD and TD groups presented body weight reduction in relation to controls, which was less accentuated in TD group. Water and food ingestion increased in the diabetic groups in relation to controls. The areas under the serum glucose curve during the GTTo of diabetic groups were higher than the controls. Physical training attenuated this elevation. The diabetic groups showed reduced PIC when compared to both control groups. The phys
Estabelecimento de condi??es de luz e temperatura para germina??o de sementes de amendoim forrageiro
Amato, Ana Laura Pereira;Maia, Fernanda Costa;Maia, Manoel de Souza;Caetano, Leandro Sebasti?o;Simioni, Silvia Bristott;Conto, Leandro de;Bonini Filho, Roberto de Moura;
Revista Brasileira de Sementes , 2007, DOI: 10.1590/S0101-31222007000300008
Abstract: the objective of this study was to compare different light and temperature regimes to establish conditions for the standard germination test of brazilian peanut (arachis pintoi krapov. & w.c. gregory). the germination tests were conducted with 200 seeds (4 x 50), using rolled paper towel. the experiment consisted of two stages. in the first, the temperatures used were 25 oc, 30 oc, 35 oc, 25-30 oc, 25-35 oc and 30-35 oc, with and without light. in the second, the alternating temperatures of 25-30 oc and 25-35 oc with light were compared with the recommended (20-30 °c with light) for peanut (arachis hypogaea l.). three statistical repetitions were used, in a completely randomized design. it was verified that the use of alternating temperatures promoted a significant decrease in the number of hard seeds because dormancy was overcome. among the treatments with constant temperature, 30 oc with light showed the highest number of normal seedlings. the temperatures of 20-30 oc and 25-30 oc, with light, were the most recommended for the germination test. daily countings of normal seedlings showed that the best day for the first counting was the 8th and for the last one the 14th day of the germination test.
Níveis de energia líquida e ractopamina para leitoas em termina??o sob conforto térmico
Moura, Mariana Souza de;Kiefer, Charles;Silva, Camilla Mendon?a;Nantes, Clariana Leon;Silva, Elizangela Alves da;Martins, Leandro Pereira;
Revista Brasileira de Zootecnia , 2011, DOI: 10.1590/S1516-35982011000900018
Abstract: this research was conducted to evaluate the net energy and ractopamine levels in the diet of finishing gilts kept under thermal comfort conditions. forty gilts were used, with initial weight of 67.4 ± 3.4 kg, distributed in a randomized blocks design in a 2 × 4 factorial scheme composed of two levels of ractopamine, 0 and 20 ppm, combined with net energy levels of 2,300; 2,424; 2,548 and 2,668 kcal/kg of feed, with five replications, considering each animal per experimental unit. the experimental period lasted 28 days. air temperature, relative humidity, black globe temperature, and black globe temperature and humidity index were 21.5 ± 2.8oc, 91.7 ± 6.8%, 21.7 ± 2.9oc and 70.1 ± 3.7, respectively. there was interaction between net energy levels and ractopamine, once the inclusion of 20 ppm of ractopamine in diets with 2,668 kcal of net energy/kg of feed resulted in a decrease in backfat thickness and consequently an increase in lean meat percentage and in carcass allowance index. the inclusion of 20 ppm of ractopamine caused an increased in daily weight gain, improved feed gain ratio, providing higher hot carcass weight and increasing the lean meat amount in carcasses. diets supplemented with 20 ppm of ractopamine improve the performance and increase meat production of finishing gilts. ractopamine is ineffective in reducing fat deposition and increasing the percentage of lean meat in the carcass of gilts fed with diets containing low concentration of energy. the level of 2,300 kcal of net energy/kg diet meets the nutritional requirement of finishing gilts under thermal comfort.
Potato clones with multiple copies of the Ryadg allele conferring resistance to PVY
Cristiana Moura Andrade,César Augusto Brasil Pereira Pinto,Silvia Regina Rodrigues de Paula Ribeiro,Leandro Santos Peixouto
Crop Breeding and Applied Biotechnology , 2009,
Abstract: Potato (Solanum tuberosum L.) breeding for PVY resistance is of utmost importance, in view of the greatdamage this disease can cause. The establishment of triplex (RyRyRyry) or quadruplex (RyRyRyRy) clones as parents facilitatebreeding work because their progenies have a high proportion of clones with the resistant allele. The purpose of this study wasto select clones with three to four copies of the Ryadg allele from a population derived from duplex (RyRyryry) crosses and toevaluate the agricultural traits. Testcrosses with a nulliplex genotype (ryryryry) were performed to identify the genotypes forthe Ryadg allele, using the SCAR RYSC3 marker. Triplex and quadruplex clones were identified by the marker, but a largernumber of progenies of the test-cross must be tested for the confirmation of their genotypes. The agricultural performance ofthe clones with the Ryadg allele was better than of the control cultivars.
Testando as hipóteses do modelo de mincer para o Brasil
Moura, Rodrigo Leandro de;
Revista Brasileira de Economia , 2008, DOI: 10.1590/S0034-71402008000400003
Abstract: many estimates of rates of return for education have been produced, based on the mincer's model. but some of the hyphoteses (linearity and separability), so that the ("mincer") school coefficient is interpreted as rate of return, are tested and rejected. when relaxing such hypotheses, we estimate the internal rates of return becker (1975) and we get biases that arrive to 14 percentile points in relation to the "mincer coefficient". thus, the magnitude of these returns is much lower than the papers based on mincer's model. in the estimates we incorporate the sample design of pnad and correct the problem of bias of sample selection.
Spirulina, exercício e controle da glicemia em ratos diabéticos
Moura, Leandro Pereira de;Gurj?o, André Luiz Demantova;Jambassi Filho, José Claudio;Mizuno, Julio;Suemi, Clara;Mello, Maria Alice Rostom de;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2012, DOI: 10.1590/S0004-27302012000100005
Abstract: objective: the objective of this study was to analyze the effect of spirulina and/or exercise training in the control of serum glucose homeostasis in diabetic rats. materials and methods: young wistar rats were induced to diabetes by intravenous alloxan administration and separated into four groups: diabetic control (dc), diabetic spirulina (ds), diabetic exercise (de) and diabetic exercise spirulina (des). results: there were no differences between groups with respect to: body weight, food intake, glucose tolerance, insulin tolerance and blood lactate concentrations during a swimming effort test. ds group showed lower insulin concentrations when compared with dc (pancreas) and de and des (serum). conclusion: the protocols of exercise and supplementation with spirulina used in the present study were not able to improve serum glucose homeostasis in diabetic rats.
Dietary restriction, caloric value and the accumulation of hepatic fat
Leandro P Moura, Gabriella A Figueredo, Natália O Bertolini, Marilia Ceccato, Jessica R Pereira, Amanda Christine S Sponton, Maria Alice R de Mello
Lipids in Health and Disease , 2012, DOI: 10.1186/1476-511x-11-2
Abstract: Therefore, the aim of the present study was to assess the effects of two non-isocaloric diets (commercial Purina? and AIN-93M), which are considered standard diets, on the accumulation of fat in the liver of rats when offered ad libitum or in a restricted amount.Thus, 40 Wistar rats (90 days old) were separated into 4 groups according to the amount of food offered (ad libitum or dietary restriction) and the type of diet (commercial diet, 3,028.0 kcal/g or AIN-93M, 3,802.7 kcal/g): animals fed the commercial Purina? diet ad libitum (AP), animals fed restricted amounts of the commercial Purina? diet (RP), animals fed the AIN-93M diet ad libitum (AD), and animals fed restricted amounts of the AIN-93M diet (RD). Dietary restriction consisted of pair-feeding the RP and RD groups with 60% of the total food consumed by the corresponding ad libitum groups.Because of its higher carbohydrate and calorie content, AIN-93M was found to accelerate weight gain, reduce glucose tolerance and peripheral insulin sensitivity, and increase the amount of fat in the liver when compared to the commercial diet. Conversely, a 40% dietary restriction assisted in weight loss without causing malnutrition, contributing to an improved glucose tolerance and higher levels of HDL cholesterol.Therefore, differences in the amount of carbohydrates and calories provided by the diet can lead to important metabolic disorders, such as impaired tolerance and accumulation of hepatic fat, and dietary restriction improves serum and tissue lipid profiles in laboratory animals.Non-alcoholic fatty liver disease (NAFLD) is characterised by the presence of fat droplets in the liver [1,2]. Lipid levels equal to or greater than 5% of an organ's weight [1,3] characterises steatosis, which may range from simple fat accumulation without any evidence of inflammation to necroinflammatory manifestations, including steatohepatitis. Steatohepatitis, in turn, may progress to fibrosis in 50% of cases, to cirrhosis in 15% of ca
Different Protocols of Physical Training: Effect on Markers of Oxidative Stress in Erythrocytes in Rats  [PDF]
Marcelo Costa-Junior, Wener Barbosa-Resende, Michel Barbosa de Araújo, Rodrigo Augusto Dalia, Leandro Pereira de Moura, Luciana Alves de Medeiros, Lucas Moreira Cunha, Eliete Luciano
Open Access Library Journal (OALib Journal) , 2018, DOI: 10.4236/oalib.1104411
Abstract:
The imbalance between oxidant molecules and antioxidant agents is characterized as oxidative stress (OS) and may lead to severe damage to the organism. In contrast, the physical training of aerobic and resistive character promotes increases of the antioxidant response, resulting in a balance and/or minimizing damage. Therefore, the objective of the study is to verify the effect of aerobic training, resistive training and concurrent training on oxidative stress of Wistar rats. Methods: To perform the experiment, 40 Wistar rats were subjected to eight weeks of training. The animals were distributed into 4 different groups: control, aerobic, resistive and concurrent (combination of aerobic and resistive protocols in the same section) an h/day, five days/week, for eight weeks. At the end of the experimental period: the antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase), biomarkers of oxidative stress (TBARs and H2O2) as well as the activity of reduced and oxidized glutathione were analyzed. Results: Regarding the markers, TBARs and H2O2 trainings were not effective in promoting significant reduction. In contrast, the three training protocols caused an increase in SOD and GSH-GPx enzymes. Conclusion: Therefore, we concluded that both training protocols promoted benefit to the organism, because they caused the increase of antioxidant enzymes without disturbance in the pro-oxidant markers.
Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil
Muraro, Lívia Saab;Caramori Júnior, Jo?o Garcia;Amendoeira, Maria Regina Reis;Pereira, Joyce Alves;Oliveira Filho, Jo?o Xavier de;Vicente, Regiane Trigueiro;Neves, Leandro Batista;Nicolau, José Leonardo;Igarashi, Michelle;Moura, Saulo Teixeira;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.1590/S1984-29612010000400012
Abstract: this report aimed to assess the seroprevalence of toxoplasma gondii infection in 708 swine matrices in nova mutum and diamantino in the state of mato grosso, central-west brazil. serum samples were examined by indirect fluorescent antibody test (ifat). it was found a seroprevalence of 12.8%, considering titers >64. therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with t. gondii.
Consumo médio e digestibilidade aparente dos nutrientes do feno de aveia (Avena sativa L.) e ervilha (Pisum sativum L.) em ovinos submetidos a dois regimes alimentares
Mizubuti, Ivone Yurika;Khatounian, Carlos Armênio;Ribeiro, Edson Luis de Azambuja;Rocha, Marco Antonio da;Silva, Leandro das Dores F. da;Moura Filho, José;Pinto, Andrea Pereira;Fernandes, Walberto Costa;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000400029
Abstract: this experiment was carried out with the objectives of determining the daily average intake (dai), apparent digestibility coefficient (adc) of oat hay and pea nutrients and to evaluate the nitrogen balance in sheep. ten wethers with average weight of 36.64 kg, alloted to suitable cages, were used. a completely randomized design with two treatments (t1= 80% oat hay + 20% pea and t2= 60% oat hay + 40% pea) and five replicates, was used. the treatments were evaluated under two feeding systems (voluntary and restrict). daily average intake of dry matter (dm) and neutral detergent fiber (ndf) did not differ between treatments.daily average intake of acid detergent fiber (adf) and crude fiber (cf) were higher for treatment one. animals submmitted to voluntary intake, showed higher nutrients average intake than the ones fed diet with 60% oat hay + 40% pea. there was no significant difference between both feeding systems to adc of dm, nitrogen free extract (nfe) and crude protein (cp). however, adc of organic matter (om), adf, ndf and cf were higher in restrict intake. there was higher adc of dm, nfe, om and cp in treatment two and there was no differences between treatments to adc and ndf and cf.
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