Objective: To profile
the differential gene expression of the KEGG Adipocytokine Signaling pathway
in omental compared to subcutaneous tissue in normal pregnancy. Study Design:
Subjects included 14 nonobese, normal glucose tolerant, healthy pregnant
women. Matched omental and subcutaneous tissue were obtained at elective
cesarean delivery. Gene expression was evaluated using microarray and validated
by RT-PCR. Differential gene expression was defined as ≥1.5 fold increase at p <
0.05. Results: Six genes were significantly downregulated with two upregulated
genes in omental tissue. Downregulation of Adiponectin and Insulin Receptor
substrate, key genes mediating insulin sensitivity, were observed with borderline
upregulation of GLUT-1. There were downregulations of CD36 and acyl-CoA Synthetase
Long-chain Family Member 1which are genes involved in fatty acid uptake and activation.
There was a novel expression of Carnitine palmitoyltransferase 1C. Conclusion:
Differential gene expression of Adipocytokine Signaling Pathway in omental
relative to subcutaneous adipose tissue in normal pregnancy suggests a
pattern of insulin resistance, hyperlipidemia, and inflammation.