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Preparation of porous microstructures on NiTi alloy surface with femtosecond laser pulses
ChunYong Liang,Yang Yang,HongShui Wang,JianJun Yang,XianJin Yang
Chinese Science Bulletin , 2008, DOI: 10.1007/s11434-008-0149-0
Abstract: Porous microstructures on Nickel-Titanium (NiTi) alloy surfaces were prepared by linearly polarized femtosecond lasers with moving focal point at a certain speed. It was found that various novel microstructures from feather-like ripples to cluster-like porous textures could be formed with increasing laser energy. Particularly, when the laser energy was 400 μJ, a periodic porous metal surface was generated. Measurement of X-ray diffraction showed that the grains on the sample surface were refined through femtosecond laser ablation processes, but the crystal structures still kept their original states. Analysis by X-ray photoelectron spectroscopy revealed that Ni/Ti on the sample surface was changed with an evident oxidization of titanium element under different laser energies. This investigation provides a new approach to improve the biocompatibility of NiTi-based implant devices.
Preparation of porous microstructures on NiTi alloy surface with femtosecond laser pulses
ChunYong Liang,Yang Yang,HongShui Wang,JianJun Yang,XianJin Yang,

科学通报(英文版) , 2008,
Abstract: Porous microstructures on Nickel-Titanium (NiTi) alloy surfaces were prepared by linearly polarized femtosecond lasers with moving focal point at a certain speed. It was found that various novel microstructures from feather-like ripples to cluster-like porous textures could be formed with increasing laser energy. Particularly, when the laser energy was 400 μJ, a periodic porous metal surface was generated. Measurement of X-ray diffraction showed that the grains on the sample surface were refined through femtosecond laser ablation processes, but the crystal structures still kept their original states. Analysis by X-ray photoelectron spectroscopy revealed that Ni/Ti on the sample surface was changed with an evident oxidization of titanium element under different laser energies. This investigation provides a new approach to improve the biocompatibility of NiTi-based implant devices. Supported by the National Natural Science Foundation of China (Project No. 50471048) and Specialized Research Fund for the Doctoral Program of Higher Education of China (Project No. 20040056016)
NeogenePaleoseismicEventsandtheShanwangBiota’sBurialintheLinquArea,ShandongProvince,China
TIAN,Hongshui,A.,J.,(Tom),VAN,LOON,*ZHANG,Zengqi,ZHANG,Shenhe,ZHANG,Banghua,L?,Mingying,LI,Fuchang,and,MA,Xuemin
地质学报 , 2015,
Abstract: SeveralpaleoseismiceventsarerecordedintheNeogeneLinquGroup,exposedintheLinquarea,ShandongProvince,China.Theeventswereinterpretedonthebasisoffieldworkandlaboratoryanalysis,whichshowedthepresenceofseismiteswithplasticallydeformedsoft-sedimentdeformationstructuresintheShanwangFormation,andofseismicvolcanicrocksintheYaoshanFormationwhichshowbrittledeformation.Theearthquake-triggeredsoft-sedimentdeformationsintheseismitesincludeloadstructures,ball-and-pillowstructures,flamestructures,pillow-likebeds,boudinagestructures,slumpfolds,syn-depositionalfaults,veinsofliquefiedsand,anddikesofliquefiedsandylime-mud.Theseismicactivityisalsoreflectedinwhatmightbecalled‘brittleseismites’;theseoriginatedwhen,undertheinfluenceofseismicvibrations,semi-consolidatedconglomeratewasshattered.Moreover,volcanicactivityisrelatedtointenseearthquakesthataffectedbasaltsintercalatedwithsandlayers;thesesuccessionsareknownas‘seismicvolcanicrocks’,whicharecharacterizedbyveinsofliquefiedsandintrudingthebasalts.Allabovetracesofpaleoseismicactivitywereleftfromonesingletimespanof4Mawithactiveseismicitythattookplace14?10Ma.Thistimespanisknownas‘theLinquNeogenePaleoseismicActivePeriod’,whichisdividedintofourpaleoseismicepisodes,whichwereresponsestotectonicextensionandbasinriftinginthisarea.ItevenincludestheactivityoftheYishuFaultZoneduringtheMioceneandtheNeogene.Theratiosoftraceelementsintheseismites,w(La)/w(Sc)andw(La)/w(Th)arehigherthantheaveragevalueoftheuppercrust,butw(Th)/w(Sc)islower;thisisgeochemicalevidenceforthebasinriftingthatresultedinahighsedimentationrate.Theintenseandfrequentpaleo-earthquakesareheldresponsiblefortherapidburialoftheShanwangBiota.Secondaryearthquake-inducedprocesses(e.g.slumpingofalakeshoreandthestronglyincreasedlacustrinesedimentationrate)contributedtotherapidburialofthebiota.
Purification and characterization of fibrinolytic enzyme from Bacillus subtilis BS-26
枯草芽孢杆菌BS-26菌株纤溶酶的性质分析及活性组分的分离纯化

Shumin Niu,Xiaojun Guo,Shuna Li,Hongshui Yuan,Baocheng Zhu,
牛术敏
,郭晓军,李术娜,袁洪水,朱宝成

微生物学报 , 2008,
Abstract: 目的]溶栓疗法是血栓性疾病安全且有效的治疗手段,从微生物中寻找溶栓药物是一种理想有效的途径,枯草芽孢杆菌(Bacillus subtilis)BS-26菌株发酵液具有很强的体外纤溶活性,本文分析了发酵液中纤溶酶的性质并对活性组分进行了分离纯化.方法]利用纤维蛋白平板法检测纤溶酶活性,利用硫酸铵分级盐析、DEAE-Sepharose Fast Flow阴离子交换层析和聚丙烯酰胺制备电泳等方法,进行分离纯化.结果]此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性,最适作用温度为42℃;最适pH值为9.0;Mg2 、Ca2 对此酶有明显的激活作用,而Cu2 能完全抑制酶的活性;174.2μg/mL的苯甲基磺酰氟、1000μg/mL的鸡卵类粘蛋白和1000μg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性,初步说明此酶属于丝氨酸蛋白酶类;体外溶纤作用表明,该酶溶解纤维蛋白的方式是直接溶解,而不是通过激活纤溶酶原.从该菌株的发酵液中获得了一种纤溶酶组分,比活力达8750 U/mg,回收率为3.2%,所获得样品纯度相对于发酵液提高了41倍,该酶在SDS-PAGE中是单肽链蛋白,分子量为32 kDa.结论]获得了一种纤溶酶的单一组分,为纤溶酶发酵产品的大规模纯化及进一步研制和开发新的溶栓药物提供重要理论依据.
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