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OALib Journal期刊

ISSN: 2333-9721

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PUB16 gene expression under abiotic stress and their putative role as an ARM repeat protein in Arabidopsis thaliana self-pollination pathway  [PDF]
María Gabriela Acosta, Miguel ángel Ahumada, Sergio Luis Lassaga, Víctor Hugo Casco
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.35079
Abstract: The armadillo repeat super-family proteins (ARM repeat super-family proteins) possess tandem armadillo repeats and have been postulated to play different roles in plant development, morphogenesis, defense, cell death, and signal transduction through hormone signalling. In The Arabidopsis Information Resource (TAIR), we found 113 loci closely related to ARM repeat family proteins. This extensive group of proteins was studied in flowers tissues by western blot using antibodies directed against the most conserved region of the ARM repeat family proteins. The amino acid residues sequences from TAIR were aligned and the resulting phylogenetic tree allows us to inferring their evolutionary relationships. The main finding was the high similarity between the gene product of PUB16 (At5g01830, A. thaliana) and ARC1 (Brassica napus). In order to search a possible role for PUB16 we carried out stress bioassays using hormonal and saline approaches. Gene expression using RT-PCR showed that some of the ARM repeat super-family proteins are expressed both under salt or hormonal stress conditions. Particularly these studies allowed to detect and semi-quantify PUB16 gene expression in normal or stress growth conditions. In this approach it was revealed that, only in presence of GA, the expression of mRNA-PUB16 became evident. To morphologically verify the increasing number of germinated pollen grain in gibberellins treated flowers, we used epi-fluorescence microscopy assay. These results suggest that PUB16 may participate in GA signaling pathway favoring self-pollination.
Cultivo in vitro de anteras de lino (Linum usitatissimum L.)
Sergio Lassaga,Adriana Bretón,Lucrecia Gieco,Héctor Milisich
Ciencia, Docencia y Tecnología , 2010,
Abstract: El cultivo de lino (Linum usitatissimum L.) es una alternativa productiva de interés para Entre Ríos y sus cualidades nutricionales hacen prever un incremento de la demanda en el mercado nacional e internacional. El mejoramiento genético es un instrumento que permite obtener nuevos genotipos adaptados a las condiciones constantemente cambiantes. El cultivo de anteras es considerado el método más eficiente de producción de plantas doble haploides de lino, y puede utilizarse como una biotecnología que asista a los programas de mejoramiento a fin de incrementar su eficiencia al permitir la obtención de líneas puras en un corto tiempo. Si bien el lino se ha mostrado recalcitrante para esta técnica, a través de la investigación que se presenta se lograron ajustes en el protocolo de cultivo de anteras in vitro, se llegó al correcto desarrollo de la etapa de callogénesis y se redujo sustancialmente la oxidación de los callos durante la etapa de regeneración.
Cultivo in vitro de anteras de lino (Linum usitatissimum L.)
Lassaga,Sergio; Bretón,Adriana; Gieco,Lucrecia; Milisich,Héctor; Dittrich,Alberto;
Ciencia, docencia y tecnolog?-a , 2010,
Abstract: flax (linum usitatissimum l.) is an important oil crop and an interesting agriculture production activity in entre ríos. its nutritional qualities provide a high expectation for an increased national and international commercial demand. crop genetic improvement is a tool that allows obtaining new genotypes adapted to constantly changing conditions. consumers look for better nutrition qualities and farmers needs new genotypes adapted to the ecological and climatic requirements. anther culture is considered the most successful method of producing doubled haploid plants in flax and it can be used in flax breeding programs to increase the efficiency and obtain lines in a short time. although flax appeared to be recalcitrant to this technology through the study here presented some adjustments in the protocols that allowed obtaining a suitable growth at callus stage and reduced in a significant manner the calluses oxidation during the regeneration stage were made.
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