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PCR in the investigation of canine American tegumentary leishmaniasis in northwestern Paraná State, Brazil
Velasquez Leonardo Garcia,Membrive Norberto,Membrive Umberto,Rodrigues Gesse
Cadernos de Saúde Pública , 2006,
Abstract: American tegumentary leishmaniasis (ATL) was studied in 143 dogs in a rural area in the county of Mariluz, northwestern Paraná State, Brazil, using direct parasite search, indirect immunofluorescence (IIF), and polymerase chain reaction (PCR). Thirty-nine dogs (27.3%) presented lesions suggestive of the disease, 5 (12.8%) of which were positive in direct parasite search and PCR (lesion), and of these 5, 4 were also positive by IIF. Of the 34 dogs with negative direct parasite search, 12 (35.3%) had PCR- positive lesions, and of these, 5 were also IIF-positive. One hundred and four dogs had no lesions, but 17/101 (16.8%) were IIF-positive. PCR in blood was positive in 10/38 (26.3%) of the dogs with lesions and in 16/104 (15.4%) of dogs without lesions. The association between PCR (lesion or blood), direct parasite search, and IIF detected 24/39 (61.5%) positive results among symptomatic dogs and 31/104 (29.8%) among asymptomatic animals. PCR was useful for diagnosing ATL, but there was no correlation between lesions, serology, and plasma PCR. Furthermore, detection of parasite DNA in the blood may indicate hematogenous parasite dissemination.
Flebotomíneos de municípios do norte do estado do Paraná, sul do Brasil
Membrive, Norberto Assis;Rodrigues, Gesse;Membrive, Umberto;Monteiro, Wuelton Marcelo;Neitzke, Herintha Coeto;Lonardoni, Maria Valdrinez Campana;Silveira, Thaís Gomes Verzignassi;Teodoro, Ueslei;
Entomología y Vectores , 2004, DOI: 10.1590/S0328-03812004000400009
Abstract: this work reports the results of sandfly collections in several places, during 2001 and 2002, in bom sucesso, jardim alegre, kaloré, londrina and sabáudia municipalities, north of the state. the sandflies catches were performed with shannon and falc?o traps in domiciliary areas, domestic animal shelters, barns and in the forest. a total of 4,019 specimens of 8 species were captured. nyssomyia whitmani predominates in all of the municipalities. the species migonemyia migonei, nyssomyia neivai and n. whitmani may be involved in the epidemiology of american tegumentary leishmaniasis in this state.
PCR in the investigation of canine American tegumentary leishmaniasis in northwestern Paraná State, Brazil
Velasquez, Leonardo Garcia;Membrive, Norberto;Membrive, Umberto;Rodrigues, Gesse;Reis, Nélio;Lonardoni, Maria Valdrinez Campana;Teodoro, Ueslei;Tessmann, Ione Parra Barbosa;Silveira, Thaís Gomes Verzignassi;
Cadernos de Saúde Pública , 2006, DOI: 10.1590/S0102-311X2006000300012
Abstract: american tegumentary leishmaniasis (atl) was studied in 143 dogs in a rural area in the county of mariluz, northwestern paraná state, brazil, using direct parasite search, indirect immunofluorescence (iif), and polymerase chain reaction (pcr). thirty-nine dogs (27.3%) presented lesions suggestive of the disease, 5 (12.8%) of which were positive in direct parasite search and pcr (lesion), and of these 5, 4 were also positive by iif. of the 34 dogs with negative direct parasite search, 12 (35.3%) had pcr- positive lesions, and of these, 5 were also iif-positive. one hundred and four dogs had no lesions, but 17/101 (16.8%) were iif-positive. pcr in blood was positive in 10/38 (26.3%) of the dogs with lesions and in 16/104 (15.4%) of dogs without lesions. the association between pcr (lesion or blood), direct parasite search, and iif detected 24/39 (61.5%) positive results among symptomatic dogs and 31/104 (29.8%) among asymptomatic animals. pcr was useful for diagnosing atl, but there was no correlation between lesions, serology, and plasma pcr. furthermore, detection of parasite dna in the blood may indicate hematogenous parasite dissemination.
Estudo das características epidemiológicas e clínicas de 332 casos de leishmaniose tegumentar notificados na regi?o norte do Estado do Paraná de 1993 a 1998
Castro, Edilene Alcantara de;Soccol, Vanete Thomaz;Membrive, Norberto;Luz, Ennio;
Revista da Sociedade Brasileira de Medicina Tropical , 2002, DOI: 10.1590/S0037-86822002000500004
Abstract: cases of cutaneous leishmaniasis (cl) in northern parana state have been reported since the 1950's, but towards the end of the 1980's there has been an increase in the number of human infections. from 1993 to 1998, a study was carried out in an attempt to define the epidemiological profile of leishmaniasis in this region. a total of 316 cases of cl were reported from 35 municipal districts and 16 imported cases. most cases of cl notified (70.8%) were in the age group of 15 to 49 years old and most of these were males (61.2%). of the 332 positive patients, 66.8% presented single classic lesions and 31.1% multiple lesions. most of these lesions were located in the lower limbs (47.7%) while 26.7% were in the arms and 16% on the face. thirty-two leishmania stocks were isolated and identified by isoenzymatic characterization using 13 enzymatic systems as leishmania (viannia) braziliensis.
Estudo das características epidemiológicas e clínicas de 332 casos de leishmaniose tegumentar notificados na regi o norte do Estado do Paraná de 1993 a 1998
Castro Edilene Alcantara de,Soccol Vanete Thomaz,Membrive Norberto,Luz Ennio
Revista da Sociedade Brasileira de Medicina Tropical , 2002,
Abstract: Casos de leishmaniose tegumentar no norte do estado do Paraná têm sido assinalados desde os anos 50, mas no final dos anos 80, houve um surto epidêmico. Para conhecer o perfil epidemiológico da leishmaniose cutanea na regi o Norte do Paraná foi feito um levantamento de casos, entre 1993 e 1998. Foram notificados 316 casos da doen a em 35 municípios paranaenses. Dezesseis casos eram importados de outros estados e do Paraguai. Os indivíduos do sexo masculino (61,2%), na faixa etária de 15 a 49 anos (70,8%) representaram a maioria dos casos de leishmaniose tegumentar. Sessenta e sete porcento dos pacientes apresentaram les es únicas, 31% les es múltiplas e 2% les es de mucosas. Num total de 367 les es analisadas 47,7% estavam localizadas nos membros inferiores, 26,7% nos membros superiores e 16% na face. Trinta e duas cepas de Leishmania isoladas foram identificadas, com base em 13 isoenzimas, como pertencentes à espécie Leishmania (Viannia) braziliensis.
Leishmaniose tegumentar americana humana e canina no Município de Mariluz, Estado do Paraná, Brasil
Lonardoni, Maria Valdrinez Campana;Silveira, Thaís Gomes Verzignassi;Alves, Waneska Alexandra;Maia-Elkhoury, Ana Nilce Silveira;Membrive, Umberto Assis;Membrive, Norberto Assis;Rodrigues, Gesse;Reis, Nélio;Zanzarini, Paulo Donizete;Ishikawa, Edna;Teodoro, Ueslei;
Cadernos de Saúde Pública , 2006, DOI: 10.1590/S0102-311X2006001200020
Abstract: an outbreak of american cutaneous leishmaniasis was reported in 2002 in mariluz, northwestern paraná state, brazil. of 38 humans who were investigated, four had healed lesions, ten showed lesions in the healing process, and 24 had active lesions. of the 126 dogs, 20 (15.9%) presented suggestive lesions and 24 (19%) had positive serology. parasites isolated from two patients and three dogs were identified as leishmania (viannia) braziliensis serodeme i. the captured sand flies were identified as nyssomyia whitmani, n. neivai, and migonemyia migonei. considering that the region where the cases occurred is similar to other old human settlements in paraná state, the environmental alterations and remaining forests facilitate the maintenance of the parasite's enzootic cycle and transmission to humans and domestic animals, thereby maintaining the endemicity of american cutaneous leishmaniasis.
Environmental and Animal Characteristics as Factors Associated with American Cutaneous Leishmaniasis in Rural Locations with Presence of Dogs, Brazil
Norberto Assis Membrive, Gesse Rodrigues, Kezia Peres Gualda, Marcos Vinícius Zandonadi Bernal, Diego Molina Oliveira, Maria Valdrinez Campana Lonardoni, Ueslei Teodoro, Jorge Juarez Vieira Teixeira, Thaís Gomes Verzignassi Silveira
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0047050
Abstract: The aim of the study was to investigate the importance of dogs, other domesticated animals and environmental characteristics as risk factors in the epidemiology of American cutaneous leishmaniasis (ACL). A retrospective survey of cases of human ACL in the last ten years and visits to homes in rural locations were carried out in the municipality of Arapongas (southern Brazil) from 2008 to 2010. ACL in humans was significantly associated with a distance of up to 25 meters from the residence to a forest area (OR 5.08; 95% CI: 1.35–21.04), undergrowth area (OR 6.80; 95% CI: 1.69–45.33) and stream (OR 5.87; 95% CI: 1.15–24.59); banana plants near the residence (OR 5.98; 95% CI: 1.49–39.84), absence of ceiling below the roof in the residence (OR 7.30; 95% CI: 1.26–158.1), the dumping of trash in the forest area (OR 26.33; 95% CI: 7.32–93.46) and presence of ACL in dogs in the surrounding area (OR 4.39; 95% CI: 1.37–13.45). In dogs, ACL was associated with a distance of 25 to 50 meters and 51 to 100 meters, respectively, from the residence to a forest area (OR 2.59; 95% CI: 1.08–5.98; OR 3.29; 95% CI: 1.64–6.62), the presence of a stream up to 25 m from the residence (OR 6.23; 95% CI: 2.34–16.54) and banana plants near the residence (OR 0.45; 95% CI: 0.25–0.80). In the locations studied in the municipality of Arapongas (Brazil), the results reveal that canine infection increases the risk of human infection by ACL and the characteristics surrounding the residence increase the risk of infection in both humans and dogs. Thus, integrated environmental management could be a useful measure to avoid contact between humans and phlebotomines.
The clinical seminar as a learning methodology: an evaluation of nursing students' views
Granero-Molina, José;Fernández-Sola, Cayetano;Adelaida María, Castro-Sánchez;Jiménez-López, Francisca Rosa;Aguilera-Manrique, Gabriel;Márquez-Membrive, Josefa;
Acta Paulista de Enfermagem , 2012, DOI: 10.1590/S0103-21002012000300019
Abstract: objective: to explore students' assessments of the clinical seminar as a complementary teaching method to the clinical practicum experience. methods: this was a qualitative study based on the hermeneutic phenomenology of gadamer. twenty-three open-ended interviews were conducted from among the 132 first-year students who attended an initial clinical practicum. we performed a qualitative analysis of the data using atlas.ti software. results: the students agreed that the clinical seminar gave them the opportunity to learn about procedures, nursing care and interpersonal relationships. they also found it very helpful when they encountered challenging stressful situations as they performed their practice, and believed it allowed them to make a connection between the theory in the classroom and the clinical practice. conclusions: these seminars can contribute to reducing levels of stress during clinical practice. they can also help students obtain significant learning from their fellows and reduce the theory-practice gap.
Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil
Scodro, Regiane Bertin de Lima;Reinhold-Castro, Kárin Rosi;Dias-Sversutti, Alessandra de Cassia;Neitzke-Abreu, Herintha Coeto;Membrive, Norberto de Assis;Kühl, Jo?o Balduíno;Silveira, Thaís Gomes Verzignassi;Teodoro, Ueslei;
Brazilian Archives of Biology and Technology , 2008, DOI: 10.1590/S1516-89132008000300007
Abstract: the purpose of this work was to verify the occurrence of leishmania in naturally infected sandflies. the insects were collected with falc?o, shannon and hp light-traps, in doutor camargo and maringá municipalities between november 2004 and october 2005. of the 11,033 sandflies collected in doutor camargo, 2,133 surviving females were dissected, particularly those of the nyssomyia neivai species (86.87%). in maringá, 136 sandflies were collected, of which 79 n. whitmani females and 1 migonemyia migonei female were dissected. the dissected insects were identified and stored in the pools of 10 specimens. the pcr was carried out on 1,190 females of n. neivai and 190 of n. whitmani from doutor camargo, and on 30 of n. whitmani from maringá, using the primers mp1l/mp3h. the natural infection by leishmania in sandflies was not confirmed by either of the methods used. the results suggested the low natural infection rate of sandflies by leishmania in these areas, corroborating other studies carried out in endemic areas of acl.
Phenetic analysis of Panstrongylus megistus Burmeister, 1835 (Hemiptera: Reduviidae: Triatominae) in the State of Paraná-Brazil
Kopp, Rogério Luiz;Thomaz-Soccol, Vanete;Klisiowicz, Débora do Rocio;Membrive, Norberto;Barata, José Maria Soares;Jurberg, José;Steindel, Mário;Kopp, Denize Cristina Trevisan;Castro, Edilene Alcantara de;Luz, Ennio;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000200012
Abstract: panstrongylus megistus is an important chagas disease vector and is said to be one of the species that might replace triatoma infestans as the main vector of that disease in brazil. the different degrees of p. megistus domiciliation in brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. thus, the present work aimed at studying the genetic variability of p. megistus in the state of paraná - south of brazil- and at comparing it with populations of the same species from five other states in brazil (sp, mg, sc, rs, se). in order to attain the proposed objective, 25 populations were studied using fifteen isoenzymatic systems (6pgd, g6pd, me1, me2, icd, pgm, gpi, got1, got2, np1, np2, dia, mpi, f, and mdh). the phenetic analysis allowed the individuation of 22 electromorphs and five zymodemes. the g6pd enzyme was the only polymorphic one presenting four electromorphs for the studied populations, all of them described for the state of paraná-br. the p. megistus populations from other states grouped with those from paraná-br, evidencing a low genetic variability in that species. despite the existing geographic barriers, sub-samples - away from one another by at most 570km - were grouped in one and the same zymodeme. the epidemiological implications of such results are discussed in the present work.
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