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OALib Journal期刊

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APPLICATION OF BIOAUGMENTATION TO SOLVE AMMONIA IN THE SEDIMENT OF THE CULTURE MEDIUM OF TIGER SHRIMP (Penaeus monodon F.) IN DIFFERENT SALINITIES
Sarjito
Journal of Coastal Development , 2009,
Abstract: Bioaugmentation was used to solve the ammonia problem, because this method is relatively safe to theenvironment. The objective of this research was to determine the influence and effectiveness ofbioaugmentation on the reduction of ammonia in the sediment in culture medium of Tiger shrimp (P.monodon). Laboratory scale experiment and splitted plot randomized design was performed. The maintreatment was probiotic epicin concentration of 0; 0.5; 1.0; and 1 ppm; as sub-treatment was the salinity(20; 25 and 30 ppt), and as a group was dayobservation (0; 2; 4 and 6). Sediment from intensive culturesystem was taken from brackish water pond of Brackishwater Aquaculture Development Project (BADP),Jepara. Sediment ammonia was analyzed using methods of Parson et al.,(1989). The research wasconducted at hatchery of Fisheries and Marine Science Faculty of Diponegoro University, Teluk Awur,Jepara. The result indicated that bioaugmentation was able to reduce ammonia concentration in thesediment. Epicin has high significantly effected on the reduction of ammonia in the sediment (p<0.01). Thecapability of epicin as a bioaugmentation started on the day of 2. Therefore, the results also showed that atime (day) was highly significant affect on the reduction of ammonia in the sediment (p<0.01). However,salinity did not affect on the reduction of ammonia in the sediment (p>0.05). The most effective epicin doseto reduce ammonia in the sediment of the culture medium of Tiger shrimp (P. monodon) was 1.5 ppm
LIPID PRODUCING MICROALGAE FROM SEVERAL ECOSYSTEMS IN WEST AND CENTRAL JAVA, INDONESIA
DWI ANDREAS SANTOSA,SULASTRI,SARJITO
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2010,
Abstract: This study is aimed to get lipid producing microalgae as feedstock for biofuel production. The microalgae were isolated from 355 collected water samples which represented many distinct ecosystems such as paddy fields, rivers, agricultural dams, ponds, swampy areas and unique ecosystem of volcano and mud-volcano craters in West- and Central Java, Indonesia. A total of 267 strains of microalgae were isolated from the samples of which 221 strains of them have capability to produce lipid. There were four promising strains that produce lipid between 14.7 – 45.7 percent dry weight in optimal condition that were identified as Chlamydomonas sp. KO-7267 and PK-7195, Chlorella sp. KS-7300 and Desmodesmus sp. BK-7291.
THE WHITE SPOT SYNDROME VIRUS (WSSV) LOAD IN Dendronereis spp.
Desrina, Sarjito, Alfabetian Harjuno Condro Haditomo, Diana Chilmawati
Journal of Coastal Development , 2012,
Abstract: The white spot syndrome virus (WSSV), the causative agent of White Spot Syndrome Disease (WSSD), is a major shrimp pathogen in Indonesia. Dendronereis spp. is a ubiquitous Polychaetes and natural food of shrimp raised in brackishwater pond in Indonesia. The objective of this research is to determine the occurrence of WSSV and the viral load in Dendronereis spp. obtained from the shrimp pond. Dendronereis spp. was obtained with PVC (10 cm in diameter) from a traditional shrimp pond in Semarang vicinity. As acomparison, healthy looking Penaeus monodon was also obtained from the same pond. The occurrence of WSSV in Dendronereis spp. was determined with 1-step and nested PCR using primer for WSSV major envelope protein, VP 28. The viral load was counted with 1-step Real Time PCR. The WSSV was detected in Dendronereis spp. with 1-step and nested PCR. The point prevalence of WSSV infection in Dendronereis spp. is 90 %. The viral load ranged from 0 to 1.9 x 104 copy of DNA/μg total DNA. The viral load in Dendronereis is comparable with that of naturally infected and at carrier state P.monodon from the same pond. This is the first report of WSSV load in naturally infected Dendronereis spp.
Phylogenetic Diversity of the Causative Agents of Vibriosis Associated with Groupers Fish from Karimunjawa Islands, Indonesia
Sarjito,O.K. Radjasa,A. Sabdono,S.B. Prayitno
Current Research in Bacteriology , 2009,
Abstract: A molecular-based study was conducted to estimate the richness of the causative agents of vibriosis associated with groupers from Karimunjawa islands, North Java Sea, Indonesia. Moribound grouper fish were collected from the cage cultures and a total of 32 isolates were isolated from external wound and kidney of groupers. Based on the repetitive sequence-based PCR (rep-PCR) and Koch postulate test, eight isolates were chosen for further sequencings. On the basis of the sequence analysis, the data showed that the causative agents are closely related with Vibrio natriegen, V. oliviceaus, V. fortis, V. alginolitycus, V. harveyi, V. parahemolitycus, V. damsela and V. carchariae, respectively. Present study highlighted the effectiveness of rep-PCR in rapid grouping and estimating the richness of the causative agents of vibriosis associated with the groupers.
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.)
Sarjito , Nestin E. W. Ningrum , Ocky Karna Radjasa and Slamet Budi Prayitno
Journal of Coastal Development , 2012,
Abstract: A molecular-based study was conducted to estimate the richness of the Vibrio on tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively. The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrioon tiger shrimp.
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