全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

Comparative Study of Two Conventional Methods Used for Coliform Enumeration from Port Harcourt Waters

DOI: 10.4236/oalib.1102500, PP. 1-5

Subject Areas: Environmental Sciences, Microbiology

Keywords: Membrane Filtration, Most Probable Number, Coliforms

Full-Text   Cite this paper   Add to My Lib

Abstract

This study compares two conventional methods: Membrane Filtration (MF) and Multiple Tube Fermentation (MTF) used in the analysis of two Port Harcourt natural water sources, Abonnema Wharf (A) and Tourist Beach (B). However, these methods are generally used in the determination of the sanitary condition of the waters and its suitability for general use. It is observed from the study that the MF gives a more reliable and precised data than that of the MTF which is time consuming, labour intensive and less precised. Bacterial isolates such as the Enterococcus faecalis not present with the use of MF method may be as a result of seasonal changes, spatial distribution of organism, and varying degree of human/animal and industrial activities/influence on the ecosystem. Studies on the effects of physiological stress especially stress by chlorination should be carried out on the waters in order to substantiate the effectiveness of each method and also identify the best conventional method to be applied during analysis of water.

Cite this paper

Nnadozie, P. C. (2016). Comparative Study of Two Conventional Methods Used for Coliform Enumeration from Port Harcourt Waters. Open Access Library Journal, 3, e2500. doi: http://dx.doi.org/10.4236/oalib.1102500.

References

[1]  APHA (1998) Standard Methods for the Examination of Water and Wastewater. 20th Edition, American Public Health Association, Washington DC. 
[2]  Tobin, R.S., Lomax, P. and Kushner, D.J. (1980) Comparison of Nine Brands of Membrane Filter and the Most-Proba- ble-Number Methods for Total Coliform Enumeration in Sewage-Contaminated Drinking Water. Journal of Applied and Environmental Microbiology, 40, 186-191.
[3]  Roohul-Amin, Syed, S.A., Zubair, A. and Jabar, Z.K.K. (2012) Microbial Analysis of Drinking Water and Water Distribution System in New Urban Peshawar. Current Research Journal of Biological Sciences, 4, 731-737.
http://maxwellsci.com/print/crjbs/v4-731-737.pdf
[4]  Ibiene, A.A. and Frank-Peterside, N. (2010) Water Quality: Physicochemical and Microbiological Standards. Essentials of Entrepreneurship, Acotec Technologies, Port Harcourt, 140-148.
[5]  Hsu, S.C. and Williams, T.J. (1982) Evaluation of Factors Affection the Membrane Filters Technique for Testing Drinking Water. Applied and Environmental Microbiology, 44, 453-460.
[6]  LUn, S. (1977) Evaluation of Methods for Detecting Coliforms and Fecal Streptococci in        Chlorinated Secondary Sewage Effluents. In: Bordner, R.H., Frith, C.F. and Winter, J.A., Ed., Proceedings of the Symposium on the Recovery of Indicator Organisms Employing Membrane Filters, Report No. EPA-600/9-77-024, US Environmental Protection Agency, Washington DC, 113-131.
[7]  Lin, S. (1973) Evaluation of Coliform Tests for Chlorinated Secondary Effluents. Journal of the Water Pollution Control Federation, 45, 498-506.
[8]  Hoffmann, D.A., Kuhns, J.H., Stewart, R.C. and Crossley, E.I. (1964) A Comparison of Membrane Filter Counts and Most Probable Numbers of Coliform in San Diego’s Sewage and Receiving Waters. Journal of the Water Pollution Control Federation, 36, 109-117.
[9]  Hydrology Project Training Module File (1999) 22 Coliforms as Indicator of Faecal Pollution.doc. Version 05/11/02, New Delhi, 1-46.
[10]  Abu, G.O. and Nnadozie, P.C. (2015) Faecal Pollution and Enteric Bacterial Distribution in Port Harcourt Waters: A Case Study of Abonnema Wharf and Tourist Beach Waters. Int. J. of Sc. Res. and Educ., 3, 4462-4472.
http://ijsae.in/ijsaeems/index.php/ijsae/article/view/967
[11]  Nnadozie, P.C. (2015) The Implication of Residual Bacterial Isolates from Port Harcourt Waters. International Journal of Environmental Monitoring and Analysis, 3, 312-316.
http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=162
[12]  US Environmental Protection Agency (1977) Manual for the Interim Certification of Laboratories Involved in Analyzing Public Drinking Water Supplies. Environmental Protection Agency publication No. 600/8-78-008. Office of Monitoring and Technical Support, US Environmental Protection Agency, Washington DC.
[13]  Cheesbrough, M. (2006) District Laboratory Practice in Tropical Countries. Part 2, 2nd Edition, Cambridge University Press Publication, South Africa, 1-434.

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133

WeChat 1538708413