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Isolation, Identification and Degradation Characteristics of Oil Degrading Bacterial Strain

DOI: 10.4236/oalib.1104016, PP. 1-12

Subject Areas: Environmental Sciences

Keywords: Degrading Bacteria, Brevibacillus Laterosporus, Oil Degrading

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Abstract

Objective: The aim of study is to obtain a strain which can effectively degrade oil. Methods: By enrichment, domestication and separation of culture from the sample which comes from Qianjiang Guanghua Oilfield, the pure culture (G-40) was selected from the medium supplemented with oil as the sole source of carbon. The G-40 was preliminarily identified and classified by morphological observation, physiological and biochemical determination and sequence analyses of 16S rDNA. Effects of temperature, pH, oil concentration, salt concentration, inoculation amount, N and P source on the degradation of the oil were studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration were optimized by orthogonal design. Result: G-40 was isolated and identified as Brevibacillus laterosporus. The orthogonal design showed that the degradation rate of oil could reach 20.81%, 24.34% and 31.15% at 10 d, 20 d and 40 d under the optimal conditions which was 35℃, initial pH 7.3, oil concentration 0.6%, salt concentration 0.5%, inoculum amount 8%, the optimal N and P was (NH4)2SO4 and K2HPO4. Conclusion: This study provides microbial resources for bioremediation of petroleum contaminated soil.

Cite this paper

Zhan, Y. , Tao, X. , He, S. , Song, S. , Xing, J. , Li, F. , Jiang, T. and Ma, L. (2017). Isolation, Identification and Degradation Characteristics of Oil Degrading Bacterial Strain. Open Access Library Journal, 4, e4016. doi: http://dx.doi.org/10.4236/oalib.1104016.

References

[1]  Holliger, C., Gaspard, S., Glod, G., Heijman, C., Schumacher, W., Schwarzenbach, R.P. and Vazquez, F. (1997) Contaminated Environments in the Subsurface and Bioremediation, Organic Contaminants. Fems Microbiology Reviews, 20, 517-523.
https://doi.org/10.1111/j.1574-6976.1997.tb00334.x
[2]  Das, N. and Chandran, P. (2011) Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotechnology Research International, 2011, Article ID: 941810.
https://doi.org/10.4061/2011/941810
[3]  Alvarez, P.J. and Vogel, T.M. (1991) Substrate Interactions of Benzene, Toluene, and Para-Xylene during Microbial Degradation by Pure Cultures and Mixed Culture Aquifer Slurries. Applied and Environmental Microbiology, 57, 2981-2985.
[4]  Ma, J., Yan, G.X., Ma, W.F., Cheng, C.M., Wang, Q.G. and Guo, S.H. (2015) Isolation and Characterization of Oil-Degrading Microorganisms for Bench-Scale Evaluations of Autochthonous Bioaugmentation for Soil Remediation. Water Air Soil Pollution, 226, 272-281.
https://doi.org/10.1007/s11270-015-2491-6
[5]  Laorrattanasak, S., Rong-sayamanont, W., Khondee, N., Paorach, N., Soonglerdsongpha, S., Pinyakong, O. and Luepromchai, E. (2016) Production and Application of Gordonia Westfalica GY40 Biosurfactant for Remediation of Fuel Oil Spill. Water Air Soil Pollution, 227, 325-337.
https://doi.org/10.1007/s11270-016-3031-8
[6]  Chu, W. and Kwan, C.Y. (2003) Remediation of Contaminated Soil by a Sol-vent/Surfactant System. Chemosphere, 53, 9-15.
https://doi.org/10.1016/S0045-6535(03)00389-8
[7]  Hamme, J.D., Singh, A. and Ward, O.P. (2003) Recent Advances in Petroleum Microbiology. Microbiology and Molecular Biology Reviews, 67, 503-549.
https://doi.org/10.1128/MMBR.67.4.503-549.2003
[8]  Medinabellver, J.I., Marin, P., Delgado, A., Rodríguezsánchez, A., Reyes, E., Ramos, J.L. and Marques, S. (2005) Evidence for In Situ Crude Oil Biodegradation after the Prestige Oil Spill. Environ-mental Microbiology, 7, 773-781,
https://doi.org/10.1111/j.1462-2920.2005.00742.x
[9]  April, T.M., Foght, J.M. and Currah, R.S. (2000) Hydrocarbon-Degrading Filamentous Fungi Isolated from Flare Pit Soils in Northern and Western Canada. Canadian Journal of Microbiology, 46, 38-49.
[10]  Alsayegh, A., Alwahaibi, Y., Albahry, S., Elshafie, A., Albemani, A. and Joshi, S. (2015). Microbial Enhanced Heavy Crude Oil Recovery through Biodegradation Using Bacterial Isolates from an Omani Oil Field. Microbial Cell Factories, 14, 141-151.
https://doi.org/10.1186/s12934-015-0330-5
[11]  Leahy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 54, 305-315.
[12]  Li, B.M., Ruan, Z.Y. and Jiang, R.B. (2007) Screen and Identification of Oil Degrading Bacteria and Community Construction. Soil and Fertilizer Sciences in China, 3, 68-72.
[13]  Zhao, B. and He, S.J. (2002) Microbiology Experiment. Science Press, Beijing.
[14]  Sawadogo, A., Otoidobiga, H.C., Nitiema, L.W., Traore, A.S. and Dianou, D. (2014) Isolation and Characterization of Hydrocarbon-Degrading Bacteria from Wastewaters in Ouagadougou, Burkina Faso. Journal of Environmental Protection, 5, 1183-1196. https://doi.org/10.4236/jep.2014.512115
[15]  Yang, Q., Wu, M.L., Cao, B.X., Cao, W.J. and Nie, M.Q. (2014) Isolation of Petroleum Degrading Strains and Determination Their Degrading and Gene Characteristics. Journal of Safety and Environment, 14, 187-192.
[16]  Liu, H., Yao, J., Yuan, Z., Yuan, Z.M., Shang, Y.F., Chen, H.L., Wang, F., Masakorala, K., Yu, C., Cai, M., Blake, R.E. and Choi, M.M.F. (2014) Isolation and Characterization of Crude-Oil-Degrading Bacteria from Oil-Water Mixture in Dagang Oilfield, China. International Biodeterioration & Biodegradation, 87, 52-59.
[17]  Chen, C., Chen, S.F., Wang, J.Y., Mao, Z.Q., Zhou, B. and Wang, B. (2014) Isolation, Identification and the Biocontrol Potential of a Strain of Brevibacillus laterosporus. Microbiology China, 41, 2275-2282.
[18]  Shida, O., Takagi, H., Kadowaki, K. and Komagata, K. (1996) Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillusgen. nov. International Journal of Systematic Bacteriology, 46, 939-946.
https://doi.org/10.1099/00207713-46-4-939
[19]  Lima, D.F., Olivia, O.M.C.D., Geris, R.M.D.S. and Barreto, L.I.S. (2017) Isolation and Selection of Fungi for Degrading Saturated Hydrocarbons, Aromatic Hydrocarbons and NSO Compounds. Open Journal of Yangtze Gas and Oil, 2, 10-26.
https://doi.org/10.4236/ojogas.2017.21002
[20]  Battu, S. and Pottabathini, V. (2015) Hydrolytic Degradation Study of Lansoprazole, Identification, Isolation and Characterisation of Base Degradation Product. American Journal of Analytical, 6, 145-155.
https://doi.org/10.4236/ajac.2015.62013

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