Objective: The aim of
study is to obtain a strain which can effectively degrade oil. Methods: By
enrichment, domestication and separation of culture from the sample which comes
from Qianjiang Guanghua Oilfield, the pure culture (G-40) was selected from the
medium supplemented with oil as the sole source of carbon. The G-40 was
preliminarily identified and classified by morphological observation,
physiological and biochemical determination and sequence analyses of 16S rDNA.
Effects of temperature, pH, oil concentration,
salt concentration, inoculation amount, N and P source on the degradation of the oil were
studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration were optimized
by orthogonal design. Result: G-40 was isolated and identified as Brevibacillus laterosporus. The orthogonal
design showed that the degradation rate of oil could reach 20.81%, 24.34% and
31.15% at 10 d, 20 d and 40 d under the optimal conditions which was 35℃,
initial pH 7.3, oil concentration 0.6%, salt concentration 0.5%, inoculum
amount 8%, the optimal N and P was (NH4)2SO4 and K2HPO4. Conclusion: This study provides microbial
resources for bioremediation of petroleum contaminated soil.
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